DMSO did not increase BrdU incorporation according to observation by microscopy. DMSO was the solvent for 20E. As a control, the same volume of DMSO (20 %) was injected and the midguts were sampled. As another control, naïve and DMSO-injected larvae were also sampled at 0 h post injection. Some samples of naïve larvae (without 20E injection) and DMSO-injected larvae were stained with secondary (2nd) antibodies only (A-B). DMSO did not enhance BrdU incorporation (E-L). Without BrdU injection or by omitting the primary (1st) antibody against BrdU, there was no signal (A-D). Bar: 50 μm. (PPT 3272 kb
