Supporting Experimental Data (.pdf) â Contains: Table AF5.1, which lists the PCR primers used. Figure AF5.1 which displays baseline expression for genes of interest in a subset of LM-MEL melanoma cell lines using qPCR. Figure AF5.2, which displays siRNA transfection efficiency; Figure AF5.3, which displays additional qPCR results showing the effects of miR-29b mimic/inhibitor transfection. Figure AF5.4 & AF5.5, which display the effects of miR-29b mimic/inhibitor transfection on LM-MEL-77 cell proliferation and outgrowth. Figure AF5.6, which displays information to aid the readerâs interpretation of melanoma spheroid data. (PDF 3182Â kb