Abstract

<p>(A-B) Subnuclear localization of DCL1 (A) and HYL1 (B) in Col-0 and <i>dbr1-2</i> root cells. Right panels show the percentage distribution of dicing bodies per cell. The X-axis represents the number of dicing bodies per cell, and the Y-axis represents the percentage of cells with the corresponding numbers. “n” represents the numbers of analyzed cells. (C) Morphological phenotypes of the <i>hyl1-2</i> and the <i>dbr1-2 hyl1-2</i> double mutant. Pictures were taken of 5-week-old plants. (D) Expression levels of indicated pri-miRNAs in Col-0, <i>dbr1-2</i>, <i>hyl1-2</i>, and <i>dbr1-2 hyl1-2</i> plants by qRT-PCR. <i>UBQ5</i> was used as an internal control and for normalization of the data. Standard deviations were calculated from three technical replicates. The results shown were reproduced with three biological replicates. Scale bars = 10 μm.</p

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