Line graph showing changes in distribution of plasma membrane labelled Lyn-Ceru compared with ER-labelled STIM1-mRFP, TM-Venus and TLP-Venus in CHO cells, post cyclohexamide treatment. CHO cells transfected with the plasma membrane labelled Lyn-Ceru and the ER labelled STIM1-mRFP, TM-Venus or TLP-Venus. Cells were imaged initially and after 1, 4 and 12 h after incubation with 10 μg/mL cyclohexamide. Y-axis shows PCC of the STIM1-mRFP, TM-Venus or TLP-Venus with Lyn-Ceru. TM: transmembrane domain TLR4, TLP: fusion of TM with signal peptidase cleavage site from human immunoglobulin K, V: Venus fluorescent protein, LC: Lyn-Ceru, STIM1: stromal interaction molecule 1. Error bars indicate s.d. Star indicates significance of p < 0.05. (PDF 238 kb
