<p>HL-1 cells were subjected to 150 min ischemia followed by 5 min of reperfusion (A); CK release to the supernatant was measured at the indicated times (B); protein carbonylation content was measured by western blot in the cell lysate before and after the 5 min reperfusion (C). Isolated rat hearts were subjected to ischemia for 30 min and 15 min reperfusion (D); CK release into the perfusate was measured during reperfusion (E); protein carbonylation content was measured by western blot at the end of reperfusion (15 min) (F).</p
