<p>HL-1 cells were subjected to 150 min ischemia followed by 5 min reperfusion, and cell extracts were obtained at the indicated times and probed for p62 by western blot (A). Isolated rat hearts were subjected to 30 min ischemia followed by 15 min reperfusion, and the heart homogenates were fractionated by differential centrifugation to yield cytosol (B, D, F) and mitochondria (C, E, G). The resulting fractions were probed for p62 (B and C), Beclin 1 (D and E) and Drp1 (F and G) by western blot.</p
