<p>Fibroblasts grown on coverslips were fixed and then stained with DAPI. (A) Scoring of fibroblast cells with micronuclei. At least 3000 cells were scored from each of P1, P2, S1, S2 cells in matched cell passage number from four independent experiments. Example of a cell with a micronucleus is shown in inset, scale bar 15 μm. (B) Representative cells showing DNA bridges connecting interphase cells. Top panel shows normal and the bottom two panels show affected interphase cells with interconnecting DNA threads. Note the bottom cell has had the DAPI/DNA (blue) signal enhanced to visualise the DNA thread. Cells were co-stained with anti-α-tubulin (red). Scale bar 5 μm. (C) Scoring of fibroblast cells with interphase interconnecting DNA threads. At least 3000 cells were scored from each of parental control (P1, P2) and RMI2 deficient siblings (S1, S2) in matched cell passage number from three independent experiments. Error bars represent standard error of the mean.</p
