<p>(A) Cell culture protocol for deriving long-term cultures of iNGNs. Astrocytes (ACs) were plated onto coverslips between 1 and 3 days before iNGNs. (B) Representative DIC microscope images of iNGNs were taken over the course of long-term co-culture with astrocytes, from 1–112 days. Ages (in days) are labeled in the upper left corners of the images. The uppermost left image shows iNGNs that are undifferentiated (no Dox). (C) Intrinsic properties of iNGNs from 4 to 70 days were measured in whole-cell patch-clamp recordings, and averaged measurements are shown for cell capacitance, resting membrane potential, and input resistance. Error bars denote SEM and numbers next to the errors bars show the number of cells recorded at each age.</p
