Induction of IFN-β and ISGs in WT and SK1^-/- mice following ic infection with DENV-2.

Abstract

<p>WT and SK1^-/- mice were ic infected with DENV, as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0169814#pone.0169814.g001" target="_blank">Fig 1</a>. RNA was isolated from infected mice brain tissues and analysed by real time qRT-PCR <b>A.</b> at 3 dpi for IFN-β, viperin, Ifi27l2a, and CXCL10. n = 8 WT and SK1^-/- DENV-infected, n = 2 WT mock and n = 3 SK1^-/- mock-infected mice; <b>B.</b> at end stage disease for IFN-β, viperin, Ifi27l2a, and CXCL10. n = 12 WT and n = 10 SK1^-/- DENV-infected, n = 7 WT mock and n = 4 SK1^-/- mock-infected mice. Data points representing non-symptomatic animals (9–14 dpi) are indicated by the half-filled symbols. Statistical analysis has been performed on symptomatic DENV-infected mice only (7–8 dpi), excluding n = 5 WT and n = 3 SK1^-/- at 9/14 dpi. Data represent average PCR values from individual mice and normalized against GAPDH by ΔCt method. Statistical significance was assessed by unpaired student <i>t</i>-test. * = p < 0.05, ** = p < 0.005, *** = p < 0.0005, **** = p < 0.00005.</p