<p>Determination of (A-B) parasite load in the spleen and the liver by limiting dilution assay and (C-D) hepatosplenomegaly in vaccinated and non-vaccinated mice 4 months post challenge with <i>L</i>. <i>infantum</i>. Results are presented as means±SD of five individual mice per group, representative of two independent experiments with similar results. *<i>p</i><0.05, **<i>p</i><0.01, ***<i>p</i><0.001 were assessed by one-way ANOVA and Tukey’s multiple comparison tests.</p