Taxonomic assignments to operational taxonomic unit

Baker, Christopher C. M.

Baker, Christopher C.M.

Billen, Johan P. J.

Billen, Johan P.J.

Frederickson, Megan E.

Martins, Dino J.

Pelaez, Julianne N.

Pierce, Naomi E.

Pringle, Anne

Christopher C. M. Baker (181637)

Dino J. Martins (3641653)

Julianne N. Pelaez (3641656)

Johan P. J. Billen (3641650)

Anne Pringle (150401)

Megan E. Frederickson (445140)

Naomi E. Pierce (111973)

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Supplement S4 from Distinctive fungal communities in an obligate African ant plant mutualism

Three ant species nest obligately in the swollen-thorn domatia of the African ant-plant Vachellia (Acacia) drepanolobium, a model system for the study of ant-defence mutualisms and species coexistence. Here we report on the characteristic fungal communities generated by these ant species in their domatia. First, we describe behavioural differences between the ant species when presented with a cultured fungal isolate in the laboratory. Second, we use DNA metabarcoding to show that each ant species has a distinctive fungal community in its domatia, and that these communities remain characteristic of the ant species over two Kenyan sampling locations separated by 190 km. Third, we find that DNA extracted from female alates of Tetraponera penzigi and Crematogaster nigriceps contained matches for most of the fungal metabarcodes from those ant species' domatia, respectively. Fungal hyphae and other debris are also visible in sections of these alates' infrabuccal pockets. Collectively, our results indicate that domatium fungal communities are associated with the ant species occupying the tree. To the best of our knowledge, this is the first record of such ant-specific fungal community-level differences on the same myrmecophytic host species. These differences may be shaped by ant behaviour in the domatia, and by ants vectoring fungi when they disperse to establish new colonies. The roles of the fungi with respect to the ants and their host plant remain to be determined

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Data from: Distinctive fungal communities in an obligate African ant-plant mutualism

Dryad Digital Repository (Duke University)

Three ant species nest obligately in the swollen-thorn domatia of the African ant-plant Vachellia (Acacia) drepanolobium, a model system for the study of ant-defence mutualisms and species coexistence. Here we report on the characteristic fungal communities generated by these ant species in their domatia. First, we describe behavioural differences between the ant species when presented with a cultured fungal isolate in the laboratory. Second, we use DNA metabarcoding to show that each ant species has a distinctive fungal community in its domatia, and that these communities remain characteristic of the ant species over two Kenyan sampling locations separated by 190 km. Third, we find that DNA extracted from female alates of Tetraponera penzigi and Crematogaster nigriceps contained matches for most of the fungal metabarcodes from those ant species' domatia, respectively. Fungal hyphae and other debris are also visible in sections of these alates' infrabuccal pockets. Collectively, our results indicate that domatium fungal communities are associated with the ant species occupying the tree. To the best of our knowledge, this is the first record of such ant-specific fungal community-level differences on the same myrmecophytic host species. These differences may be shaped by ant behaviour in the domatia, and by ants vectoring fungi when they disperse to establish new colonies. The roles of the fungi with respect to the ants and their host plant remain to be determined.denoised sequencesFASTA file of sequences following processing of .sff files with Ampliconnoise in QIIME. Each sequence is labelled as >sample.no_sequence.no, where sample.no corresponds to the original .sff file names, and sequence.no is sequential within each sample.amplnoiseout.fna.tar.gzmetadata fileMetadata file describing samples in this study.kenya_mapfile.txtmetadata file keyKey to metadata file describing samples in this study.kenya_mapfile_key.txtraw data filesRaw 454 data files (.sff format). Data are already demultiplexed, with one file for each sample in study, as described in the accompanying metadata file.Baker_2016_rawdata.tar.gzFunding provided by: National Science FoundationCrossref Funder Registry ID: http://dx.doi.org/10.13039/100000001Award Number: SES-075048

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NEUROSURGERY ENTHUSIASTIC WOMEN SOCIETY

https://figshare.com/articles/Supplement_S4_from_Distinctive_fungal_communities_in_an_obligate_African_ant_plant_mutualism/4556014

Supplement S4 from Distinctive fungal communities in an obligate African ant plant mutualism

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NEUROSURGERY ENTHUSIASTIC WOMEN SOCIETY