Additional file 2: Figure S2. of Functional role of ALK-related signal cascades on modulation of epithelial-mesenchymal transition and apoptosis in uterine carcinosarcoma
(A) Two independent Hec251 cell lines stably overexpressing ALK (H251-ALK#8 and #16) and mock cells were seeded at low density and monitored for growth. The cell numbers presented are means ± SDs. P0, P3, P5, and P7: 0, 3,5, and 7 days after passage. (B) Western blot analysis of expression of cyclin A, p21waf1, and p27kip1 at P6 of cell growth in stable ALK-overexpressing cell lines. (C) The pNF-κB reporter construct was transfected into H251-ALK#16 cells treated with 2.5 ng/ml TGF-β1 or 50 ng/ml HGF for 48 h. Relative activity was determined based on arbitrary light units of luciferase activity normalized to pRL-TK activity. The activities of the reporter plus the effector relative to that of the reporter plus empty vector are shown as means ± SDs. The experiment was performed in duplicate. (D) Various promoter constructs were used for evaluating transcriptional regulation of the ALK promoter by TNF-α. (E) The pNF-κB reporter construct, together with the ALK expression vector, were transfected into Ishikawa cells. (TIF 843 kb
