Additional file 1: Figure S1. of Metabolite toxicity determines the pace of molecular evolution within microbial populations

Abstract

The number of non-synonymous mutations in coding regions in the evolved clones for each experimental evolution condition. Horizontal bars and P-values indicate the outcomes from two-sample Wilcoxon rank-sum tests. A star indicates a P-value <0.05. Data are presented as Tukey box-plots. Figure S2. The relative fitness of the evolved clones calculated from competition with the ancestor at the conditions of the evolution with an inital frequency of 50% of evolved cultures. There is no significant difference in the increase in fitness between pH 7.5 and pH 6.5 (Wilcoxon rank sum test, P > 0.4, n1 = n2 = 8). Table S1. Mutations that accumulated in clones evolved at pH 7.5. Table S2. Mutations that accumulated in clones evolved at pH 6.5. Table S3. Strains and plasmids used in this study. Table S4. Oligonucleotide PCR primers used for cloning the egfp or echerry gene into the pUC18T-mini-Zn7T-LAC-Gm plasmid. (PDF 239 kb