<p>Analysis by MALDI-TOF MS of hydatid fluid from a bovine cyst (A), as well as of chymotrypsin-affinity purified fractions from the same sample (B) and from the supernatant of cultured immature adults (C). Signals whose m/z values could derive from the <i>Eg</i>KUs are indicated (MH<sup>+</sup> predicted for mature <i>Eg</i>KU-3 and <i>Eg</i>KU-8 are: 6406.8 and 6520.9, respectively). Note that the signals putatively corresponding to the <i>Eg</i>KUs are significantly enriched in the eluate from the affinity matrix. The identity of <i>Eg</i>KU-3 and <i>Eg</i>KU-8 purified from cyst fluid was subsequently confirmed by peptide mass fingerprinting (see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006169#ppat.1006169.s001" target="_blank">S1 Dataset</a> and the text for further details), as previously described [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006169#ppat.1006169.ref003" target="_blank">3</a>].</p
