Additional file 10. Evidence for the existence of Pfs38 complex by sedimentation analysis. Glycerol gradient fractionation of Plasmodium schizont extract using 5 to 45% glycerol gradient and immunoblotting of glycerol gradient fractions using anti-Pfs38, anti-Pfs41, anti-Pfs12, anti-GLURP, anti-PfMSP-165 and anti-SERA5 antibodies. Note the co-sedimentation of Pfs41, Pfs38, Pfs12, GLURP, PfMSP-165 and Sera-5 in fraction 5
