<p>The oregano, <i>Origanum onites</i> L., essential oil (EO) was tested in laboratory behavioural bioassays for repellent activity against <i>Amblyomma americanum</i> (L.) and <i>Aedes aegypti</i> (L.). The <i>O. onites</i> EO was characterised using GC-FID and GC-MS. Carvacrol (75.70%), linalool (9.0%), <i>p</i>-cymene (4.33%) and thymol (1.9%) were the most abundant compounds. At a concentration of 0.413 mg oil/cm<sup>2</sup> of filter paper, <i>O. onites</i> EO repelled 100% of the ticks tested and at 0.103 mg oil/cm<sup>2</sup> of filter paper, 66.7% of the ticks were repelled. At 0.075 mg oil/cm<sup>2</sup> filter paper, thymol repelled 66.7% of the ticks compared to 28.7% by carvacrol at that same concentration. Against <i>Ae. aegypti</i>, <i>O. onites</i> EO was repellent at the minimum effective dosage (MED) of 0.011 (±0.00) mg/cm<sup>2</sup> in the cloth patch assay compared to the reference control, <i>N</i>,<i>N</i>-dimethyl-3-methylbenzamide (DEET) with a MED = 0.007 ± (0.003) mg/cm<sup>2</sup>.</p
