<p>Fecal protein samples from Galphai2<sup>-/-</sup> mice, Galphai2<sup>+/-</sup> mice, and wild-type littermates were subjected to immunoblotting for detection of trypsin-2. (A) Equal amounts of fecal protein were loaded from Galphai2<sup>-/-</sup> mice with early signs of colitis, along with samples from Galphai2<sup>+/-</sup> mice and wild-type littermates of similar ages. (B) Fecal protein samples from Galphai2<sup>-/-</sup> mice at different stages of colitis showed a tendency towards higher trypsin levels with advanced disease. The boxplots show densitometric quantitation of blots where trypsin-2 levels of Galphai2<sup>-/-</sup> mice were depicted relative to the mean value of the healthy wild-type littermates (A) or the pre-symptomatic Galphai2<sup>-/-</sup> mice (B). a.u. = arbitrary units.</p
