<p>Intestinal BBM vesicles (1 mg protein/ml in 50 mM sodium maleate buffer, pH 6.0), from control animals were incubated with varying concentrations (0–2.0 mM) of NaNO<sub>2</sub> at 37°C in a total reaction volume of 1.0 ml. At different time intervals after the addition of NaNO<sub>2</sub>, aliquots were removed from the reaction mixture and assayed for enzyme activity. Results are expressed relative to enzyme activity in untreated BBM vesicle samples kept on ice which served as the control. Results of a typical experiment are shown here. (A) Leucine aminopeptidase (LAP) (B) γ-glutamyl transferase (GGT) (C) alkaline phosphatase (ALP) (D) sucrase.</p
