<p>(<b>A, B</b>) A549 cells were plated onto glass coverslips and the following day replaced with media containing NB-DNJ (200μM) or left untreated. Forty-eight hours later, cells were chilled to 4°C on ice, then rVSV-SFTSV was bound by centrifugation (1200xg, 30’, 4°C). Following centrifugation, media was replaced with pre-warmed media (37°C) and the cells placed in a 37°C incubator for 20 minutes before fixation in 2% paraformaldehyde for 10 minutes. Cells were then immunostained for viral antigen (anti-VSV M, red), cellular markers (green), and nuclei stained with DAPI (blue). Images are representative from at least 3 independent experiments. (<b>A</b>) A549 cells co-stained for rVSV-SFTSV (red) and early endosome marker EEA1 (green). (<b>B</b>) A549 cells co-stained for rVSV-SFTSV (red) and <i>trans</i>-Golgi marker TGN46 (green). Boxes indicate zoomed-in regions. Scale bar represents 5μm.</p
