Visualization of TRE5-A<sup>bsr</sup> integrations at tRNA genes.

Abstract

<p>The 42 tRNA gene families and their individual copy numbers are shown as white squares. The colors indicate the association of a distinct tRNA gene copy with a TRE5-A or TRE5-A<sup>bsr</sup> element. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0175729#pone.0175729.s001" target="_blank">S1 Fig</a> for the distribution of individual tRNA genes on the six chromosomes. Gray squares indicate mitochondrial tRNA genes. (<b>A</b>) Display of tRNA gene loci associated with a TRE5-A element. (<b>B</b>) TRE5-A<sup>bsr</sup>-targeted tRNA genes identified in the tDNA primer library prepared after 20 generations (20G) or 100 generations (100G) of cell culture. Light red and dark red squares indicate tRNA genes identified only in the 20G or 100G library, respectively. Bright red squares indicate tRNA genes found in both libraries. (<b>C</b>) TRE5-A<sup>bsr</sup>-targeted tRNA genes found in the adapter primer library prepared after 100 generations of cell culture (100G).</p

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