V předkládané diplomové práci, kterou jsem vypracoval ve spolupráci s VŠB – TU Ostrava a společností Siemens s.r.o., se věnuji návrhu upevnění sedadel k boční stěně hrubé stavby kolejového vozidla. Práce souvisí s projektem výstavby metra ve městě Riyadh. Hlavními částmi této práce jsou variantní návrh upevnění pomocí tzv. cantileveru, rozpracování zvoleného řešení, pevnostně deformační analýza a kontrola šroubového spoje. Součástí práce je také teoretický rozbor norem a vyhlášek souvisejících s návrhem sedadlových systémů v kolejových vozidlech.In the presented master´s thesis, which I developed in cooperation with VŠB – TU Ostrava and company Siemens s.r.o., I designed variant Fastening of a Seats to the side wall of Rail Vehicle Frame. Thesis related with project Metro Riyadh. The main parts of this thesis are variant design of cantilever, Fastening of a seats, stress – strain analysis and check of bolted connection. Creation of drawing documentation is another part.347 - Katedra částí a mechanismů strojůvýborn

Ali Khodayari (3995480)

Costas Maranas (1310631)

Daniel Olson (2916260)

Evert Holwerda (3455144)

Jilai Zhou (612821)

Lee Lynd (3453692)

Satyakam Dash (3995477)

Biotechnology for Biofuels

Bayer, Adam

DSpace at VSB Technical University of Ostrava

Design of a Seat Mounting with a Rail Vehicle Frame

Additional file 3. The core kinetic model k-ctherm118 with list of regulations and kinetic parameters as well as fermentation yields for 19 C. thermocellum mutants

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MOESM3 of Development of a core Clostridium thermocellum kinetic metabolic model consistent with multiple genetic perturbations

Abstract Background Clostridium thermocellum is a Gram-positive anaerobe with the ability to hydrolyze and metabolize cellulose into biofuels such as ethanol, making it an attractive candidate for consolidated bioprocessing (CBP). At present, metabolic engineering in C. thermocellum is hindered due to the incomplete description of its metabolic repertoire and regulation within a predictive metabolic model. Genome-scale metabolic (GSM) models augmented with kinetic models of metabolism have been shown to be effective at recapitulating perturbed metabolic phenotypes. Results In this effort, we first update a second-generation genome-scale metabolic model (iCth446) for C. thermocellum by correcting cofactor dependencies, restoring elemental and charge balances, and updating GAM and NGAM values to improve phenotype predictions. The iCth446 model is next used as a scaffold to develop a core kinetic model (k-ctherm118) of the C. thermocellum central metabolism using the Ensemble Modeling (EM) paradigm. Model parameterization is carried out by simultaneously imposing fermentation yield data in lactate, malate, acetate, and hydrogen production pathways for 19 measured metabolites spanning a library of 19 distinct single and multiple gene knockout mutants along with 18 intracellular metabolite concentration data for a Δgldh mutant and ten experimentally measured Michaelis–Menten kinetic parameters. Conclusions The k-ctherm118 model captures significant metabolic changes caused by (1) nitrogen limitation leading to increased yields for lactate, pyruvate, and amino acids, and (2) ethanol stress causing an increase in intracellular sugar phosphate concentrations (~1.5-fold) due to upregulation of cofactor pools. Robustness analysis of k-ctherm118 alludes to the presence of a secondary activity of ketol-acid reductoisomerase and possible regulation by valine and/or leucine pool levels. In addition, cross-validation and robustness analysis allude to missing elements in k-ctherm118 and suggest additional experiments to improve kinetic model prediction fidelity. Overall, the study quantitatively assesses the advantages of EM-based kinetic modeling towards improved prediction of C. thermocellum metabolism and develops a predictive kinetic model which can be used to design biofuel-overproducing strains

Satyakam Dash

Ali Khodayari

Jilai Zhou

Evert K. Holwerda

Daniel G. Olson

Lee R. Lynd

Costas D. Maranas

Directory of Open Access Journals

Development of a core Clostridium thermocellum kinetic metabolic model consistent with multiple genetic perturbations

Additional file 2. The genome-scale model iCth446 with GPRs and metabolite information, list of TICs

MOESM2 of Development of a core Clostridium thermocellum kinetic metabolic model consistent with multiple genetic perturbations

Clostridium thermocellum is a Gram-positive anaerobe with the ability to hydrolyze and metabolize cellulose into biofuels such as ethanol, making it an attractive candidate for consolidated bioprocessing (CBP). At present, metabolic engineering in C. thermocellum is hindered due to the incomplete description of its metabolic repertoire and regulation within a predictive metabolic model. Genome-scale metabolic (GSM) models augmented with kinetic models of metabolism have been shown to be effective at recapitulating perturbed metabolic phenotypes.
In this effort, we first update a second-generation genome-scale metabolic model (iCth446) for C. thermocellum by correcting cofactor dependencies, restoring elemental and charge balances, and updating GAM and NGAM values to improve phenotype predictions. The iCth446 model is next used as a scaffold to develop a core kinetic model (k-ctherm118) of the C. thermocellum central metabolism using the Ensemble Modeling (EM) paradigm. Model parameterization is carried out by simultaneously imposing fermentation yield data in lactate, malate, acetate, and hydrogen production pathways for 19 measured metabolites spanning a library of 19 distinct single and multiple gene knockout mutants along with 18 intracellular metabolite concentration data for a Δgldh mutant and ten experimentally measured Michaelis–Menten kinetic parameters

Dash, Satyakam

Khodayari, Ali

Zhou, Jilai

Holwerda, Evert K

Olson, Daniel

Lynd, Lee

Maranas, Costas

Dartmouth Digital Commons (Dartmouth College)

English

Development of a Core Clostridium Thermocellum Kinetic Metabolic Model Consistent with Multiple Genetic Perturbations

Additional file 1. The genome-scale model iCth446 with GPRs and metabolite information in COBRA toolbox compatible SBML format

MOESM1 of Development of a core Clostridium thermocellum kinetic metabolic model consistent with multiple genetic perturbations

https://figshare.com/articles/MOESM3_of_Development_of_a_core_Clostridium_thermocellum_kinetic_metabolic_model_consistent_with_multiple_genetic_perturbations/4964012

Design of a Seat Mounting with a Rail Vehicle Frame

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DSpace at VSB Technical University of Ostrava

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Dartmouth Digital Commons (Dartmouth College)

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