Expression of adherens junction-associated proteins in control and Afadin-deleted dorsal forebrains at E13.5. (A, B) Western blot analysis of E13.5 dorsal forebrain extracts show a strong reduction of Afadin expression in mutant, but not of intercellular junctions-associated proteins α-catenin, N-cadherin, β-catenin, p120catenin, Z0-1, or any cadherin when compared to control (mean ± s.d. Unpaired t-test.; n = 3 to 8 animals). (TIF 2373 kb