Variance in single-cell data from newborn mouse hindbrain and cerebellum and 2D cluster representation of replicates. Related to Fig. 4. (a) Plots of principal components 1–18 of the 4366 cell transcriptomes show variance in many principal components. Colors correspond to tSNE plot in Fig. 4b. (b) 2D representation of experimental replicates in each cell population. tSNE plot from Fig. 4b with each cell now coloured by experimental replicate. Note that cells from the two biological replicates are unevenly represented in the different clusters, likely reflecting dissection differences and varying proportions of hindbrain to cerebellar tissue. (c) We identified a subtype of myelinating glia, probably Schwann cells from cranial nerves entering the hindbrain (cluster 11, Fig. 4b). These cells express myelin protein zero (Mpz) and other genes for myelin formation (proteolipid protein 1, Plp1) and Mbp (Fig. 4b) but do not express oligodendrocyte markers such as Bcas1 or Olig1 (Fig. 4b). (PDF 255 kb
