<p>RMF and HFF cells were either grown in the presence or absence of 50 μM LY294002 for 2 hours prior to infection and throughout the duration of infection. Cells were collected at 1, 3, 6 hpi, lysed, fractionated, immunoblotted, then probed for pAkt (S473) and total Akt. These results are representative of 3 independent experiments.</p