<p>RMF and HFF cells were grown in the presence or absence of LY294002 for 2 hours prior to infection and the treatment was continued throughout the infection for 24 hours. <b>(A)</b> Cell lysates were collected at 24 hpi and the effect of LY294002 on phosphorylation of Akt (S473) was assessed. <b>(B)</b> B virus titers in infected (MOI 5) RMF/HFF cells with or without LY294002 treatment. <b>(C)</b> B virus infectious titers in infected (MOI 0.2) RMF/HFF cells with or without LY294002 treatment. <b>(D)</b> Relative fold-reduction in infectious virus titers in RMF and HFF cells treated with LY294002. All results are representative of 3 independent experiments.</p