Additional file 1: Figure S1. of Nanospan, an alternatively spliced isoform of sarcospan, localizes to the sarcoplasmic reticulum in skeletal muscle and is absent in limb girdle muscular dystrophy 2F
nSPN mRNA transcripts are predominant in skeletal and heart muscles. RT-PCR was performed on cDNA isolated from various human tissues (skeletal muscle, testis, colon, heart, brain, breast, and bone marrow) using a forward primer in exon 1 and a reverse primer in exon 4, as illustrated in Fig. 1c. PCR products were obtained for μSPN (179 bp) and nSPN (90 bp). RT-PCR performed without template DNA is shown as a negative control (neg. ctrl). Molecular size markers are indicated on the left. (PDF 348 kb