Impact of early neutrophil infiltration in parasite killing and lesion development in hamsters infected with <i>L</i>. <i>V</i>. <i>panamensis</i>.

Abstract

<p>(A), Parasite burden in the cutaneous lesion of hamsters treated to inhibit neutrophil recruitment with either a polyclonal anti-serum (Ab) (6h before infection) or 200mg/kg cyclophosphamide (cycloph.) (24h before infection) or treated to increase neutrophil recruitment with 25 μg fMLP (6h before infection), evaluated at 6h post-infection; (B), Parasite burden in lesion of hamsters treated to inhibit neutrophil with cyclophosphamide or treated to recruit neutrophils with fMLP, evaluated at 19 days p.i. Determined in skin sections stained with H&E at 100X magnification. Number of cells = proportion of cells x total number of cells / 100 (determined in a standard area of 0.21mm<sup>2</sup> at 100X magnification). (C-D), Lesion size and severity of the skin ulcer and necrosis followed during 19 days of infection. *p<0.05; **p<0.01, with reference to control group, n = 8 hamsters per group.</p

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