Relative quantification (RQ) values of mouse beta-actin, GAPDH and 18S endogenous controls in C9-BAC mouse cortex across different age groups are shown (A). Absolute copy number of human C9ORF72 transcripts per microliter in C9-BAC mice as determined by digital droplet PCR (B), one-way ANOVA (p < 0.001) and Bonferroni’s multiple comparison test was performed between neonatal (0wks) and the remaining age groups, significance is indicated by p < 0.05 * and p < 0.01 **. (JPEG 238 kb
