Aka Gündüz'ün Cumhuriyet'te tefrika edilen Bu Toprağın Kızı adlı romanıTelif hakları nedeniyle romanın tam metni verilememiştir

Annemarie Matthes (121250)

Bent Larsen Petersen (167354)

Birger Lindberg Møller (534683)

Carl Erik Olsen (598310)

Henrik Clausen (117282)

Mika Zagrobelny (534681)

Mikael Kryger Jensen (4108801)

Stefan Pentzold (534680)

Søren Bak (463065)

Catabolism of the cyanogenic glucosides linamarin and lotaustralin. Enzymes catalysing the respective reactions are shown and the β-glucosidase as key enzyme is highlighted in gre

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Figure S1 from Spatial separation of the cyanogenic β-glucosidase ZfBGD2 and cyanogenic glucosides in the haemolymph of Zygaena larvae facilitates cyanide release

Representative Western blot against His-tag of β-glucosidases expressed in Sf9 cell

Figure S3 from Spatial separation of the cyanogenic β-glucosidase ZfBGD2 and cyanogenic glucosides in the haemolymph of Zygaena larvae facilitates cyanide release

Pentzold, Stefan

Jensen, Mikael Kryger

Matthes, Annemarie

Olsen, Carl Erik

Petersen, Bent L

Clausen, Henrik

Møller, Birger Lindberg

Bak, Søren

Zagrobelny, Mika

Copenhagen University Research Information System

English

Spatial separation of the cyanogenic β-glucosidase ZfBGD2 and cyanogenic glucosides in the haemolymph of Zygaena larvae facilitates cyanide release

Low molecular weight compounds are typically used by insects and plants for defence against predators. They are often stored as inactive β-glucosides and kept separate from activating β-glucosidases. When the two components are mixed, the β-glucosides are hydrolysed releasing toxic aglucones. Cyanogenic plants contain cyanogenic glucosides and release hydrogen cyanide due to such a well-characterized two-component system. Some arthropods are also cyanogenic, but comparatively little is known about their system. Here, we identify a specific β-glucosidase (ZfBGD2) involved in cyanogenesis from larvae of Zygaena filipendulae (Lepidoptera, Zygaenidae), and analyse the spatial organization of cyanide release in this specialized insect. High levels of ZfBGD2 mRNA and protein were found in haemocytes by transcriptomic and proteomic profiling. Heterologous expression in insect cells showed that ZfBGD2 hydrolyses linamarin and lotaustralin, the two cyanogenic glucosides present in Z. filipendulae. Linamarin and lotaustralin as well as cyanide release were found exclusively in the haemoplasma. Phylogenetic analyses revealed that ZfBGD2 clusters with other insect β-glucosidases, and correspondingly, the ability to hydrolyse cyanogenic glucosides catalysed by a specific β-glucosidase evolved convergently in insects and plants. The spatial separation of the β-glucosidase ZfBGD2 and its cyanogenic substrates within the haemolymph provides the basis for cyanide release in Z. filipendulae. This spatial separation is similar to the compartmentalization of the two components found in cyanogenic plant species, and illustrates one similarity in cyanide-based defence in these two kingdoms of life

Additional file S2 Alignments of BGDs and GBAs from Spatial separation of the cyanogenic β-glucosidase ZfBGD2 and cyanogenic glucosides in the haemolymph of Zygaena larvae facilitates cyanide release

Finci, Hüseyin Avni

eResearch@Ozyegin

Bu Toprağın Kızı - Aka Gündüz [Hüseyin Avni Finci]  Tefrikanın bulunduğu süreli yayın: Cumhuriyet   Tefrikanın bölüm sayısı: 18 Tefrikanın başlangıç tarihi ve süreli yayının sayısı: 20 Nisan 1927, 1057 Tefrikanın bitiş tarihi ve süreli yayının sayısı: 9 Mayıs 1927, 1078  Tefrikanın bölümlerine ilişkin bilgiler: Bölüm Sayı Tarih Sayfa 1  1057 18 Nisan 1927 3 2  1058 19 Nisan 1927 3 3  1059 20 Nisan 1927 3 4  1060 21 Nisan 1927 3 5  1062 23 Nisan 1927 3 6  1063 24 Nisan 1927 3 7  1064 25 Nisan 1927 3 8  1065 26 Nisan 1927 3 9  1066 27 Nisan 1927 3 10  1067 28 Nisan 1927 3 11  1069 30 Nisan 1927 3 12  1071 2 Mayıs 1927 3 13  1072 3 Mayıs 1927 3 14  1073 4 Mayıs 1927 3 15  1074 5 Mayıs 1927 3 16  1076 7 Mayıs 1927 3 17  1077 8 Mayıs 1927 3 18  1078 9 Mayıs 1927 3  