<p>Total RNA and total protein were isolated from wild-type HL60, HL60/ATRA cell lines and subjected to real-time PCR (a) and SDS-PAGE gel electrophoresis/western blot analysis (b). Expression of Nanog, Oct-4, SOX-2, CD133 and CD123 mRNA and protein was then examined. HL60/ATRA cells showed higher expression of CD133, CD123, and Nanog than HL60 cells. Data are expressed as the mean ± SEM from five independent experiments (*p, 0.05).</p
