Checkerboard assay. (a) Image of a microchip where the right half contains negative control master mix (NTC wells, n = 2520) and the left half contains lambda DNA master mix master (Positive wells, n = 1024) and negative control master mix (Test wells, n = 1496) in a checkerboard pattern. (b) Number of Test wells with signal, number of NTC wells with signal, and calculated misalignment rate for 11 MSNDs and 19 microchips. (PDF 1288 kb