(A) MTS assay showing no significant difference in cell proliferation in CASC15(S) over expressing NALM6 cells. B) PI staining of CASC15(S) over expressing NALM6 cells, showing no difference in the stages of cell cycle. C) FACS analysis of peripheral bleeds from the mice 4–20 weeks after bone marrow transplantation showing GFP positive cells as a percentage in the control and Casc15 overexpression mice. Initial GFP positivity in the engrafted bone marrow was similar in both groups. (D) Complete blood counts (CBC) of control and Casc15 overexpression mice at the week of 20 from the time of retro orbital injections. E) FACS analysis of Hardy fractions showing overall decreased B-cell fractions in Casc15 overexpression mice at 27 weeks after transplantation. (F-G) FACS analysis of LIN- and LSK+ cells from the control and Casc15 over expression mice showing no difference in those two populations. (H) Methylcellulose Colony Formation assay showing reduced number of colonies in BM cells with enforced expression of human CASC15. (I) FACS analysis of percentage of GFP+, B220+ and CD11b + cells in the spleen at the week of 16 after transplantation. Black circles, control mice; brown squares, Casc15-expressing mice. Data are represented as individual data points and a mean (bar). Statistical comparisons were completed using a two-tailed T-test; p < 0.05 (*); p < 0.01 (**); p ≤ 0.0005 (***). (PDF 89 kb
