(A) CASC15 expression is higher in RUNX1 translocated patients respect to other B-ALL subtypes with aberrancies related to chromosome 21. Probe 229280_s_at, HGU133 plus 2 Affymetrix (DS-ALL, Down Syndrome) (1- way ANOVA, p < 0.01). (B) CASC15 expression is higher in RUNX1 translocated patients respect to other AML subtypes. Probe TC06000136.hg.1 HTA 2.0 Affymetrix. (1- way ANOVA, p < 0.01). Comparisons were made using a two-tailed T-test, statistically significant differences are denoted as follows: ** P < 0.01. Source of data for (A) and (B): two datasets deposited in NCBI’s Gene Expression Omnibus database (GEO) (N = 102 ALL, N = 85 AML; GSE17459 and GSE75461) [25, 26]. (C) Diagram showing 5′ and 3′ RACE product aligned with Ref sequence obtained from the UCSC genome browser. 5′ RACE primers are shown in blue. Unannotated exons are shown in yellow. (D-E) Gel showing 5 and 3′ RACE products. (F) Kaplan Meier survival analysis for two patient groups (high and low CASC15 expressers) shows that low CASC15 expression shows a trend towards worse overall survival (Log Rank Test, P-value, n.s. p = 0.18). The two groups were dichotomized based on two step cluster analysis using SPSS software. (G) Schematic showing the exon-intron structure of the two isoforms of CASC15 (H) Schematic showing the exon-intron structure of the mouse Casc15. (I-K) RT-qPCR data showing expression of CASC15 in cytoplasm and nuclear fractionations of from REH (I), RS4;11 (J) and 697 cell lines (K). Abbreviations: WCL (whole cell lysate), C (cytoplasmic fraction), and N (nuclear fraction). GAPDH and CELF4 were used as positive controls for cytoplasmic and nuclear-localized mRNAs, respectively [5]. 1 and 2 are biological replicates. (PDF 671 kb
