OsISA1 expression patterns in different organs and at different stages of seed development using qRT-PCR analysis. (a) Transcript levels decreased in seed (10 DAF) of the sug-h mutant. (b) OsISA1 expression in 10 and 20 DAF seeds decreased in the sug-h mutant. All data are mean ± SD (n = 3). Statistical significance was determined using Student’s t-test (*P < 0.05, **P < 0.01). WT, wild-type rice (Hwacheong); DAF, days after flowering. (PDF 51 kb