<p><b>A</b>) Enzymatic dissociation of 11.5dpc limb buds with dispase releases more hindlimb cells than forelimb cells (p = 0.0021, n = 6). <b>B</b>) When micromass cultures are challenged after 1 hour of adhesion to the cell culture substrate, fewer hindlimb cells are left in the culture than forelimb cells (p = 0.011, n = 4 pools of >4 limbs each, normalised for limb number). *p<0.05, **p<0.01, ***p<0.001, Student’s <i>t</i>-test. <b>C–K;</b> Cell mixing experiments, 1:1 mix of <i>Prx1eGFP</i> hindlimb cells/wild type forelimb cells (<b>C–E</b>), <i>Prx1-eGFP</i> forelimb/wild type forelimb (<b>F–H</b>) and <i>Prx1-eGFP</i> hindlimb/wild type hindlimb (<b>I–K</b>) cells were stained for GFP protein (green) and nuclei (blue, DAPI).</p
