<p><b>(A)</b> HU affects PEV within the mating type locus. Diagram illustrates the location of <i>ade6</i><sup>+</sup> insertion at the mating type locus, between the centromere-proximal boundary and the <i>mat2-P</i> gene (strain AP144—<i>mat_L(BglII)</i>::<i>ade6</i><sup>+</sup>). Dark green box indicates a normal silencing domain. <b>(D)</b> HU treatment affects PEV induced by <i>cenH</i> at an ectopic site. Diagram illustrates the genetic construct of the strain (LW75- <i>cenH- ade6</i><sup><i>+</i></sup>). <i>cenH</i> and <i>ade6</i><sup><i>+</i></sup>are inserted in the <i>ura4</i><sup><i>+</i></sup> locus, replacing the majority of the <i>ura4</i><sup><i>+</i></sup> ORF. <b>(B-C, E-F)</b> For each strain, cell suspensions of a predominantly white colony (B, E) and red colony (C, F) are treated with HU as labeled for 24 hours, washed and plated onto YE+4S solid media, and incubated at 25<sup>°</sup>C for 5 days. The red and white colonies are counted respectively, with 300–400 total/per plate. The error bars are 1 SD of percentages for four plates. <i>p</i> values are calculated by Fisher exact test.</p
