<p>a) Targeting the VTA with AAV vectors. Mice were infused into the VTA with AAV-Cre-GFP vectors. After 2–3 weeks, mice were perfused and brains were sectioned for immunohistochemistry. Anti-GFP staining identifies infected neurons, while anti-TH staining identifies DA neurons. High magnification (60X) images show Cre-GFP expression in TH(+) and TH(-) neurons in VTA, indicated by one or two arrows, respectively. IPN: interpeduncular nucleus, ML: medial lemniscus. b) Cre recombinase expression in VTA GABA neurons. Slices from the infection shown in (a) were stained with anti-GAD65/67 antibodies. Native GFP signal was imaged without anti-GFP staining.</p
