Frequency of favorable alleles of the molecular markers linked to previously mapped stripe rust and stem rust resistance genes/QTL in the Ethiopian wheat accessions. (DOCX 16Â kb

Bedada Buta (4334101)

Kebede Muleta (4334104)

Matthew Rouse (3582596)

Michael Pumphrey (4093771)

Sheri Rynearson (4093774)

Xianming Chen (75957)

BMC Plant Biology

Comparison of different association test models using Quantile-Quantile (Q-Q) plots using BLUP values of stripe rut IT and DS across all environments. The Q-Q plot determines the magnitude of the deviation of the observed association between the markers and response to stripe rust from the expected null hypothesis of no association. Under the assumption of no association between the SNPs and the traits, a large inflation of observed P values from the expected P values indicates spurious associations. Only few true SNP-trait associations are expected to deviate from the null hypothesis. (DOCX 95Â kb

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Additional file 4: of Characterization of molecular diversity and genome-wide mapping of loci associated with resistance to stripe rust and stem rust in Ethiopian bread wheat accessions

Genomic regions significantly associated with field resistance of the Ethiopian bread wheat accessions to Puccinia striiformis f. sp. tritici at marker wise PÂ <Â 0.005 in at least four of the eight environments. (XLSX 15Â kb

Additional file 5: of Characterization of molecular diversity and genome-wide mapping of loci associated with resistance to stripe rust and stem rust in Ethiopian bread wheat accessions

Pearsonâs correlation coefficients among stripe rust infection type (IT) and disease severity (DS) in each location and season. (XLSX 12Â kb

Additional file 2: of Characterization of molecular diversity and genome-wide mapping of loci associated with resistance to stripe rust and stem rust in Ethiopian bread wheat accessions

Infection types and disease severity observed on Ethiopian wheat cultivars and landraces tested with races of Puccinia striiformis f. sp. tritici and Puccinia graminis f. sp. tritici at seedling stage and evaluated under field conditions. Allele report for markers linked to previously known stripe rust and stem rust resistance genes and SNP markers significantly associated with the resistance to both diseases (identified in this study) are summarized. (XLSX 50Â kb

Additional file 3: of Characterization of molecular diversity and genome-wide mapping of loci associated with resistance to stripe rust and stem rust in Ethiopian bread wheat accessions

Additional file 6: of Characterization of molecular diversity and genome-wide mapping of loci associated with resistance to stripe rust and stem rust in Ethiopian bread wheat accessions

Virulence/avirulence formula of Puccinia striiformis f. sp. tritici isolates used for seedling resistance screening of the Ethiopian wheat accessions. (XLSX 10Â kb

Additional file 1: of Characterization of molecular diversity and genome-wide mapping of loci associated with resistance to stripe rust and stem rust in Ethiopian bread wheat accessions

Abstract Background The narrow genetic basis of resistance in modern wheat cultivars and the strong selection response of pathogen populations have been responsible for periodic and devastating epidemics of the wheat rust diseases. Characterizing new sources of resistance and incorporating multiple genes into elite cultivars is the most widely accepted current mechanism to achieve durable varietal performance against changes in pathogen virulence. Here, we report a high-density molecular characterization and genome-wide association study (GWAS) of stripe rust and stem rust resistance in 190 Ethiopian bread wheat lines based on phenotypic data from multi-environment field trials and seedling resistance screening experiments. A total of 24,281 single nucleotide polymorphism (SNP) markers filtered from the wheat 90 K iSelect genotyping assay was used to survey Ethiopian germplasm for population structure, genetic diversity and marker-trait associations. Results Upon screening for field resistance to stripe rust in the Pacific Northwest of the United States and Ethiopia over multiple growing seasons, and against multiple races of stripe rust and stem rust at seedling stage, eight accessions displayed resistance to all tested races of stem rust and field resistance to stripe rust in all environments. Our GWAS results show 15 loci were significantly associated with seedling and adult plant resistance to stripe rust at false discovery rate (FDR)-adjusted probability (P) <0.10. GWAS also detected 9 additional genomic regions significantly associated (FDR-adjusted P < 0.10) with seedling resistance to stem rust in the Ethiopian wheat accessions. Many of the identified resistance loci were mapped close to previously identified rust resistance genes; however, three loci on the short arms of chromosomes 5A and 7B for stripe rust resistance and two on chromosomes 3B and 7B for stem rust resistance may be novel. Conclusion Our results demonstrate that considerable genetic variation resides within the landrace accessions that can be utilized to broaden the genetic base of rust resistance in wheat breeding germplasm. The molecular markers identified in this study should be useful in efficiently targeting the associated resistance loci in marker-assisted breeding for rust resistance in Ethiopia and other countries

Kebede T. Muleta

Matthew N. Rouse

Sheri Rynearson

Xianming Chen

Bedada G. Buta

Michael O. Pumphrey

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Characterization of molecular diversity and genome-wide mapping of loci associated with resistance to stripe rust and stem rust in Ethiopian bread wheat accessions

https://figshare.com/articles/Additional_file_2_of_Characterization_of_molecular_diversity_and_genome-wide_mapping_of_loci_associated_with_resistance_to_stripe_rust_and_stem_rust_in_Ethiopian_bread_wheat_accessions/5280562

Additional file 6: of Characterization of molecular diversity and genome-wide mapping of loci associated with resistance to stripe rust and stem rust in Ethiopian bread wheat accessions

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