<p><b>A:</b> Profiles of the Dps binding sites obtained in two experiments (indicated) for the genomic region with three operons of ribosomal genes (running window of nine 35 bp bins). Genes are represented by blue horizontal arrows; magenta lines show ribosomal operons. Vertical arrows mark locations of inverted repeats (if longer than 7 bp). <b>B:</b> Band shift assays performed for indicated genomic loci. The regulatory region of the <i>dps</i> gene was used as a positive control for all band shift assays in this study. Fragment from the <i>lacZ</i> coding sequence was used as a reference gene for qRT-PCR experiments. Positioning of primers for amplification (<b>F</b> and <b>R</b>) is indicated in panel <b>A</b> of this figure, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0182800#pone.0182800.g006" target="_blank">Fig 6A</a> (for the <i>dps</i> regulatory region) and in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0182800#pone.0182800.s003" target="_blank">S3 Fig</a> (for <i>rpoA</i> and <i>lacZ</i>). <b>C:</b> Changes in the expression efficiency of selected genes in response to <i>dps</i> deletion. Primers used for reverse transcription and consecutive PCR are designated as RT and PCR, respectively, here and all other figures. Expression levels were estimated based on 3 and 5 biological samples (3–18 technical repeats in each) for <i>rpoA</i> and <i>rpoD</i>, respectively. Error bars show an average deviation. Statistical significance was assessed using Student’s t-test.</p
