Additional file 14: of RNA-sequence data normalization through in silico prediction of reference genes: the bacterial response to DNA damage as case study

Abstract

Correlations of gene expression changes for 19 selected genes. Log2 ratios derived from five normalization methods (RPKM, UQ, TMM, DESeq2, and moose 2 ) are compared to qRT-PCR measurements for 30-to-0-min (upper panel) and 90-to-0-min comparisons (lower panel). R, Pearson’s rho. (TIFF 655 kb