Clinical characteristics of the 33 type 2 diabetic patients according to drug treatment and of 60 non-diabetic subjects. Table S2. Average and promoter DNA methylation according to diabetes medication, first comparing other oral medication such as Sitagliptin or Glimepiride + metformin to only metformin therapy, and second excluding all subjects that were receiving Sitagliptin or Glimepiride. Table S3. Clinical characteristics of subjects from the Kuopio Obesity Surgery Study. Table S4. DNA sequences of the primers used for pyrosequencing. Figure S1. DNA methylation of SLC22A3 and SLC47A1 in hepatocytes cultured in vitro after 8 h of metformin (0.5 mM), insulin plus metformin, insulin (100 nM) or glucose treatment in Huh-7 cells (n = 4). Means and standard deviations are shown, and paired t test was used for the analysis. Figure S2. Gene expression levels of the three metformin transporter genes in the human liver (A) (n = 42); gene expression of transporter genes according to diabetes medication after adjusting for age, sex and non-alcoholic steatohepatitis (NASH) (B). ns: no significant. (DOCX 213 kb
