Rarefaction curves of OTUs from amplicon samples collected from the five hot springs in Eritrea. The number of OTUs is plotted in relation to sub-sampled sequence datasets size (number of reads). The first letters of the sample names refer to the five hot springs (A = Akwar, E = Elegedi, G = Garbanabra, J = Gelti and M = Maiwooi), while the second letters are for sample types (A = Microbial mat, S = wet sediment, and W = water). (TIFF 1023 kb