<p>A new 2-alkylhydroquinone glucoside, 1-<i>O</i>-<i>β</i>-d-glucopyranosyl-1,4-dihydroxy-2-((<i>E</i>) 2-oxo-3-butenyl)benzene (<b>1</b>), in addition to nine known compounds were isolated from the aerial parts of <i>Phagnalon saxatile</i> (L.) Cass. (Asteraceae). Their structures were identified based on spectroscopic methods including 1D and 2D NMR, mass spectrometry (HR-ESI-MS), UV spectral analyses and by comparison with literature data. The cytotoxic activity of three isolated compounds (<b>1–3</b>) was evaluated against fibrosarcoma (HT1080), human lung cancer (A549) and breast cancer (MCF7) cell lines.</p
