Effect of ATP on substrate targeting of the Mus81 complex by RFC. (A) Mus81-Mms4 (0.4 nM) was incubated with 3′ flap substrate (4 nM) in the presence or absence of ΦX174 virion circular ssDNA (0.25 nM), ATP (1 mM), and increasing concentrations of RFC (5, 12.5, 25, 50 nM). Reactions were incubated at 37 °C for 30 min and analyzed. (B) Quantification of the data in A from three independent experiments. *Raw data provided in Additional file 2. (PDF 1432 kb
