Abstract

<p>(A) Immunoblot for NF-κBp65 in the nuclear protein of colonic epithelium. Lamin A/C was used as a loading control. The picture is representative of four independent experiments. (B) Immunohistochemical staining for NF-κBp65 in the tissues. (original magnification ×200). NF-κBp65 was detected in the nucleus of the epithelial cells in the DSS group, but this was completely blocked in the DSS plus nanoparticle curcumin group. (C) Immunostaining of NF-κBp65 in HT-29 cells. HT-29 cells were stimulated with TNF-α (100ng/ml) in the presence or absence of nanoparticle curcumin (10μM) for 15 minutes. NF-κB p65, green fluorescence; nucleus, DAPI (blue). (D) The effect of nanoparticle curcumin on IκBα phosphorylation in response to TNF-α. HT-29 cells were stimulated with TNF-α (100 ng/ml) in the presence or absence of nanoparticle curcumin (0μM, 10μM, or 50μM) for 15 minutes, and then lysed with lysis buffer. Lysates were subjected to immunoblot analysis. GAPDH were used as loading control. The data represent four independent experiments.</p

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