<p>Three essential oils (EOs) were isolated from the peel of citrus fruits <i>Citrus reticulata</i> L.<i>, Citrus reticulata chinase</i> Blanco and <i>Citrus sinensis</i> (L.) Osbeck (Family: Rutaceae) and evaluated against <i>Culex pipiens</i> L.1758 (Family: Culicidae)<i>.</i> Chemical composition indicated that the EOs were rich in essential phytochemicals including hydrocarbons, monoterpenes and sesquiterpenes. These constituents revealed some variability among the oils displaying interesting chemotypes limonene (35–51%), 1<i>R</i>-α-pinene (1.04–2.5%), γ-terpinene (0.46–5.65%) and sabinene (0.51–5.42%). The toxicity proved that <i>C. sinensis</i> oil had more effect than <i>C. reticulata chinase</i> and <i>C. reticulata</i> oils against larvae (LC<sub>50</sub> = 15.35, 16.11 and 32.84 mg/L, respectively). However, <i>C. reticulate</i> was the most active as fumigant against adults (LC<sub>50</sub> 2.74 μL/L air). The <i>in vivo</i> effect on acetylcholine esterase (AChE), carboxyl esterase (CbE), acid phosphatase (ACP), alkaline phosphatase (ALP), adenosine triphosphatase (ATPase) and glutathione-S-transferase (GST) were also demonstrated. To the best of our knowledge, this is the first report about the chemical composition and mosquitocidal activity of <i>C. reticulata chinase</i> essential oils. Conclusively, the tested essential oils could be used as eco-friendly alternatives in mosquitoes control programme.</p
