Additional file 4. Population of H. pylori Maesot strains by STRUCTURE. The H. pylori strains from 24 Thai, 20 Hmong, 15 Karen and 7 Thai-Chinese were analyzed by STRUCTURE with K from 8â15. Each horizontal bar represent one strain, and the color in a line are proportional to the probabilities that the strain belong to each population. Each color square represented the strain from Thai, Hmong, Karen and Thai-Chinese. The H. pylori Maesot strains were hspEAsia, hpAsia2 and hpEurope. Interestingly, H. pylori Hmong and Thai-Chinese strains were located in hspEAsia while H. pylori Karen strains were belonged to hpAsia2. Thai H. pylori strains were located in three population

Junko Akada (618874)

Muhammad Miftahussurur (615312)

Phawinee Subsomwong (829936)

Ratha-korn Vilaichone (793897)

Rumiko Suzuki (187579)

Thawee Ratanachu-ek (793899)

Tomohisa Uchida (212893)

Varocha Mahachai (793901)

Yoshio Yamaoka (187580)

Gut Pathogens

Background There are few studies analyzed concurrently the prevalence and genotypes of Helicobacter pylori infection with the ancestor origins from different ethnics, especially with including minority groups. We recruited a total of 289 patients in MaeSot, Thailand (154 Thai, 14 Thai-Chinese, 29 Karen and 92 Hmong ethnics). The virulence genes and genealogy of the strains were determined by PCR-based sequencing. Results Based on culture and histology/immunohistochemistry, the prevalence of H. pylori infection was 54.5% (158/289). Among 152 isolates cultured, the East-Asian-type cagA was predominant genotype among strains from Hmong, Thai-Chinese and Thai (96.0% [48/50], 85.7% [6/7] and 62.7% [47/75], respectively), whilst majority of strains from Karen had Western-type cagA (73.3% [11/15]). Patients infected with the East-Asian-type cagA strains had significantly higher activity and intestinal metaplasia in the antrum and activity in the corpus than those with Western-type cagA (P = 0.024, 0.006 and 0.005, respectively). The multilocus sequencing typing analysis discriminated that most strains from Hmong and Thai-Chinese belonged to hspEAsia (92.0 and 85.7%, respectively), whereas strains from Karen predominantly possessed hpAsia2 (86.7%) and strains from Thai were classified into hspEAsia (45.2%) and hpAsia2 (31.1%). Conclusions Helicobacter pylori genotypes were relatively different among ethnic groups in Thailand and were associated with the source of ancestor even living in a small rural town. Caution and careful check-up are required especially on Hmong ethnic associated with high prevalence of virulence genotypes of H. pylori

Phawinee Subsomwong

Muhammad Miftahussurur

Ratha-korn Vilaichone

Thawee Ratanachu-ek

Rumiko Suzuki

Junko Akada

Tomosiha Uchida

Varocha Mahachai

Yoshio Yamaoka

Universitas Airlangga Repository

Helicobacter pylori virulence
genes of minor ethnic groups in
North Thailand
by Muhammad Mif tahussurur
Submission date: 26-Apr-2019 09:29PM (UTC+0800)
Submission ID: 1119647219
File name: 13099_2017_Article_205.pdf  (1.4M)
Word count: 9113
Character count: 46858











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Helicobacter pylori virulence genes of minor ethnic groups in North Thailand

Abstract Background There are few studies analyzed concurrently the prevalence and genotypes of Helicobacter pylori infection with the ancestor origins from different ethnics, especially with including minority groups. We recruited a total of 289 patients in MaeSot, Thailand (154 Thai, 14 Thai-Chinese, 29 Karen and 92 Hmong ethnics). The virulence genes and genealogy of the strains were determined by PCR-based sequencing. Results Based on culture and histology/immunohistochemistry, the prevalence of H. pylori infection was 54.5% (158/289). Among 152 isolates cultured, the East-Asian-type cagA was predominant genotype among strains from Hmong, Thai-Chinese and Thai (96.0% [48/50], 85.7% [6/7] and 62.7% [47/75], respectively), whilst majority of strains from Karen had Western-type cagA (73.3% [11/15]). Patients infected with the East-Asian-type cagA strains had significantly higher activity and intestinal metaplasia in the antrum and activity in the corpus than those with Western-type cagA (P = 0.024, 0.006 and 0.005, respectively). The multilocus sequencing typing analysis discriminated that most strains from Hmong and Thai-Chinese belonged to hspEAsia (92.0 and 85.7%, respectively), whereas strains from Karen predominantly possessed hpAsia2 (86.7%) and strains from Thai were classified into hspEAsia (45.2%) and hpAsia2 (31.1%). Conclusions Helicobacter pylori genotypes were relatively different among ethnic groups in Thailand and were associated with the source of ancestor even living in a small rural town. Caution and careful check-up are required especially on Hmong ethnic associated with high prevalence of virulence genotypes of H. pylori

Tomohisa Uchida

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