Junko Akada (618874)

Muhammad Miftahussurur (615312)

Phawinee Subsomwong (829936)

Ratha-korn Vilaichone (793897)

Rumiko Suzuki (187579)

Thawee Ratanachu-ek (793899)

Tomohisa Uchida (212893)

Varocha Mahachai (793901)

Yoshio Yamaoka (187580)

Gut Pathogens

Background There are few studies analyzed concurrently the prevalence and genotypes of Helicobacter pylori infection with the ancestor origins from different ethnics, especially with including minority groups. We recruited a total of 289 patients in MaeSot, Thailand (154 Thai, 14 Thai-Chinese, 29 Karen and 92 Hmong ethnics). The virulence genes and genealogy of the strains were determined by PCR-based sequencing. Results Based on culture and histology/immunohistochemistry, the prevalence of H. pylori infection was 54.5% (158/289). Among 152 isolates cultured, the East-Asian-type cagA was predominant genotype among strains from Hmong, Thai-Chinese and Thai (96.0% [48/50], 85.7% [6/7] and 62.7% [47/75], respectively), whilst majority of strains from Karen had Western-type cagA (73.3% [11/15]). Patients infected with the East-Asian-type cagA strains had significantly higher activity and intestinal metaplasia in the antrum and activity in the corpus than those with Western-type cagA (P = 0.024, 0.006 and 0.005, respectively). The multilocus sequencing typing analysis discriminated that most strains from Hmong and Thai-Chinese belonged to hspEAsia (92.0 and 85.7%, respectively), whereas strains from Karen predominantly possessed hpAsia2 (86.7%) and strains from Thai were classified into hspEAsia (45.2%) and hpAsia2 (31.1%). Conclusions Helicobacter pylori genotypes were relatively different among ethnic groups in Thailand and were associated with the source of ancestor even living in a small rural town. Caution and careful check-up are required especially on Hmong ethnic associated with high prevalence of virulence genotypes of H. pylori

Phawinee Subsomwong

Muhammad Miftahussurur

Ratha-korn Vilaichone

Thawee Ratanachu-ek

Rumiko Suzuki

Junko Akada

Tomosiha Uchida

Varocha Mahachai

Yoshio Yamaoka

Universitas Airlangga Repository

Helicobacter pylori virulence
genes of minor ethnic groups in
North Thailand
by Muhammad Mif tahussurur
Submission date: 26-Apr-2019 09:29PM (UTC+0800)
Submission ID: 1119647219
File name: 13099_2017_Article_205.pdf  (1.4M)
Word count: 9113
Character count: 46858











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Helicobacter pylori virulence genes of minor ethnic groups in North Thailand

Tomohisa Uchida

Crossref

Abstract Background There are few studies analyzed concurrently the prevalence and genotypes of Helicobacter pylori infection with the ancestor origins from different ethnics, especially with including minority groups. We recruited a total of 289 patients in MaeSot, Thailand (154 Thai, 14 Thai-Chinese, 29 Karen and 92 Hmong ethnics). The virulence genes and genealogy of the strains were determined by PCR-based sequencing. Results Based on culture and histology/immunohistochemistry, the prevalence of H. pylori infection was 54.5% (158/289). Among 152 isolates cultured, the East-Asian-type cagA was predominant genotype among strains from Hmong, Thai-Chinese and Thai (96.0% [48/50], 85.7% [6/7] and 62.7% [47/75], respectively), whilst majority of strains from Karen had Western-type cagA (73.3% [11/15]). Patients infected with the East-Asian-type cagA strains had significantly higher activity and intestinal metaplasia in the antrum and activity in the corpus than those with Western-type cagA (P = 0.024, 0.006 and 0.005, respectively). The multilocus sequencing typing analysis discriminated that most strains from Hmong and Thai-Chinese belonged to hspEAsia (92.0 and 85.7%, respectively), whereas strains from Karen predominantly possessed hpAsia2 (86.7%) and strains from Thai were classified into hspEAsia (45.2%) and hpAsia2 (31.1%). Conclusions Helicobacter pylori genotypes were relatively different among ethnic groups in Thailand and were associated with the source of ancestor even living in a small rural town. Caution and careful check-up are required especially on Hmong ethnic associated with high prevalence of virulence genotypes of H. pylori

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Additional file 5. Phylogenetic tree of cagA of hspEAsia strains from Bhutan, Hmong strains of Maesot and Hmong strains of Vietnam. We analysed 10 Bhutanese strains, 9 Hmong strains from Vietnam and 18 from Hmong Maesot with East-Asian-type cagA and hspEAsia. There was no different among cagA sequences among three group

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                     Helicobacter pylori virulence genes of minor ethnic groups in North Thailand

Additional file 1: Table S1 Primers for detecting various virulence genes. Table S2 Relationship between gastric mucosal status by upstream EPIYA sequences and ethnic group. Table S3 Gastric mucosal status by vacA genotypes. Table S4 The distribution of H. pylori population, cagA and vacA m genotypes and ethnicit

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                     Helicobacter pylori virulence genes of minor ethnic groups in North Thailand

Additional file 3. Histological score according to genotype of cagA in Karen ethnic. H. pylori harbored with East-Asian-type cagA (n = 4) induced activity and intestinal metaplasia higher than Western-type cagA (n = 11) genotype (P < 0.05, Mann–Whitney U test

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                     Helicobacter pylori virulence genes of minor ethnic groups in North Thailand

Institut national de la statistique et des études économiques (France). Auteur du texte

Gallica, Bibliotheque Numerique