The construction and expression of pColdI-rLi protein plasmid. (a) The construction of pColdI-rLIP plasmid. M, DL10000 Marker; 1, pColdTMIDNA; 2, pColdTMIDNA (BamH Ι/Hind III); 3, PCR products of rLIP; 4, rLi protein(BamH Ι/Hind III); 5, pColdI-rLIP; 6, Double digestion products of pColdI-rLIP. (b) Total protein was separated by 12% SDS-PAGE under reducing conditions and stained with Coomassie Brilliant Blue R-250. Lane M, low molecular weight protein maker; Lane 1, crude lysate pre-induction; Lane 2, crude lysate post-induction; Lane 3, purified LIP. (PDF 234 kb
