Comparison of the osteogenic differentiation potentials of hTMSCs cultured under serum-free medium versus those of hTMSCs cultured under serum-containing medium.
<p>Under osteogenic conditions, alkaline phosphatase staining (x 400) of hTMSCs cultured in serum-free medium (left) and serum-containing medium (right) demonstrated similar levels of alkaline phosphatase expression, as assessed visually. The mRNA expression levels of osteocalcin, Runt-related transcription factor 2, and type I collagen in hTMSCs (B) were perceived by RT-PCR. hTMSCs cultured under serum-free medium showed higher expression of osteogenic differentiation markers versus hTMSCs cultured under serum-containing medium.</p
